This video-game is based off the movie
- Spider-Man takes on the mask and must face Uncle Ben's killer.
- Mysterio's first Super-Thug is unleashed upon the city and Spidey must stop the terror of... The Beetle!
- Spider-Man must stop a robbery at the daily bugle as Mysterio's thugs are flooding the building led by Herman Schultz!
- Spider-Man infiltrates Mysterio's lair, as Mysterio is seen turning Schultz into... The Shocker!
- Spider-Man battles The Shocker but Shocker is accompanied by the super-thug Electro!
- Spidey loses sight of Shocker and Mysterio but defeats Electro. Spider-Man must find them by defeating Mysterio's super-thug army led by the Rhino! (note: also in the super-thug army is Silvermane, Hydro-Man, Sand-Man, and Boomerang.)
- Spidey gets to a bus full of henchmen accompanied by the latest super-thug, The Scorpion!
- The bus crashes into an abbandoned ware-house where The Scorpion gets accompanied by The Shocker!
- Spidey escapes but is being chased down by The Shocker.
- Spidey finally faces off with Mysterio, who has figured out Spider-Man's true identity and kidnapped both Felicia Hardy and Aunt May!
- The Venom symbiote is a DLC only suit.
- The 2099 suit is also DLC.
- Noir suit is DLC also, and the Scarlet Spider is the last DLC suit.
- Morbius is a DLC boss battle as Mysterio's last super-thug before The Scorpion.
- "The Battle of Venom!" is a DLC mini-game where Spider-Man must chase down Venom.
- Beetle, Electro, Rhino, Silvermane, Hydro-Man, Sand-Man, Boomerang, Morbius The Living Vampire, and Venom don't appear in the movie.
- In the movie Spider-Man battles Shocker before entering Mysterio's Lair, in the game Shocker's debut is in Mysterio's lair.
- In the movie Shocker's debut doesn't have any other super-thugs but just regular criminals.